《植物生理学报》 2015, 51(6): 977-982

烟草幼苗期耐低钾突变体的筛选及验证

徐丽娟¹, 王倩², 郑春花¹, 隋炯明¹, 刘光亮¹, 刘贯山^2,*

¹青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109; ²中国农业科学院烟草研究所, 山东青岛266101

通信作者：刘贯山；E-mail: liuguanshan2002@163.com；Tel: 0532-88703168

摘　要：

为了探讨筛选烟草耐低钾突变体的最优条件, 以烟草野生型品种‘中烟100’为材料, 研究了不同钾离子浓度对其根生长的影响, 确定了在培养基中筛选耐低钾突变体的适宜钾离子浓度为0.075 mmol•L^-1。然后以EMS诱变处理获得的‘中烟100’ M₂代种子为材料, 进行了大规模的耐低钾突变体的筛选, 初步筛选出了99个M₂代候选材料。选取50个材料继续进行了验证, 其中22个M₃代突变体经复选鉴定弯根率≥75%, 这些突变体在低钾培养基上长势明显好于对照, 且绝大多数突变体的单位鲜重叶片的钾含量明显高于对照。研究结果可为烟草耐低钾突变体的筛选提供依据。

关键词：烟草; EMS诱变; 耐低钾突变体; 弯根; 筛选

收稿：2015-04-13   修定：2015-04-30

资助：中国烟草总公司项目(110201201004JY-04和11020130-1005JY-05)。

Screening and Identification of Low K⁺ Tolerant Mutants at the Seedling Stage of Nicotiana tabacum

XU Li-Juan¹, WANG Qian², ZHENG Chun-Hua¹, SUI Jiong-Ming¹, LIU Guang-Liang¹, LIU Guan-Shan^2,*

¹College of Life Science, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong 266109, China; ²Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China

Corresponding author: LIU Guan-Shan; E-mail: liuguanshan2002@163.com; Tel: 0532-88703168

Abstract:

In order to determine the optimal condition for screening of K⁺ tolerant mutants, a wild type variety, Nicotiana tabacum cultivar ‘Zhongyan 100’ was selected to analyze the effect of different concentrations of K⁺ on the growth of roots in this experiment and the optimal K⁺ concentration for screening of K⁺+ tolerant mutants was considered to be 0.075 mmol•L^-1. Then, M₂-generation mutagenic seeds of ‘Zhongyan 100’ treated by EMS were selected for screening of K⁺ tolerant mutants. Finally, 99 M₂-generation candidate individuals were obtained. We selected 50 individuals to further examine their tolerance to low K⁺. The results showed that 22 of 50 individuals were obtained with a higher root-bending rate (≥75%) and their growth vigor and K⁺ content of fresh weight per gramme was obviously better than that of the mutagenic parent, ‘Zhongyan 100’ in low K⁺ medium. This study can provide basis for screening of low K⁺ tolerant mutants in N. tabacum.

Key words: Nicotiana tabacum; EMS mutagenesis; low K⁺ tolerant mutant; root-bending; screening